牛FABP3转基因小鼠的遗传及表达特性研究

doi:10.11843/j.issn.0366-6964.2015.10.007

摘要/Abstract

摘要：

旨在研究和分析牛FABP3基因在小鼠体内遗传和表达特性，对培育优质高产转基因肉牛新品种具有重要意义。本研究利用前期获得的4只FABP3转基因小鼠，通过制定的4组交配方案获得子代小鼠，并利用PCR、实时荧光定量PCR和组织原位表达技术对转基因牛FABP3基因小鼠的子代遗传特征，以及FABP3基因在G0代和G1代小鼠心、肝、肾、骨骼肌组织的表达情况进行了检测。RT-PCR检测结果表明，在G0代转基因小鼠中，牛FABP3基因能够表达；牛FABP3基因在转基因小鼠中可以遗传，G1代转基因小鼠平均阳性率达68.4%；相对定量PCR结果表明，转基因小鼠总FABP3基因在G1代小鼠心肌和骨骼肌中表达量相对于阴性个体有不同程度的增加；组织原位检测表明，在G0代个体中表达量较高，G1代个体中虽能看到绿色荧光，但略有降低。本研究表明，牛FABP3基因存在于小鼠基因组中，并能够被表达，且可以遗传给后代，这为后期进一步研究牛FABP3基因对小鼠骨骼肌脂肪含量的影响奠定坚实基础。

Abstract:

The study aimed to analyze the genetic characteristic and expression of bovine FABP3 gene in transgenic mice，which will play a key role in culturing the breeds that produced higher yield and improved quality by transgenic method.Four FABP3 transgenic mice were used，G1 generation mice were obtained by 4 mating schmes.The genetic characteristic and expression of bovine FABP3 in heart，liver，kidney and skeletal muscle of transgenic mice were investigated in this study by RT-PCR and fluorescent in situ hybridization.The results of RT-PCR showed that bovine FABP3 gene could be identified in G0 transgenic mice by RT-PCR and propagated to G1 offspring，too.The positive rate was 68.4% in G1 and FABP3 gene expression levels in heart，skeletal muscle of transgenic mice were much higher than that of negative controls by real-time PCR.Furthermore，the fluorescent in situ hybridization results showed that bovine FABP3 gene could be expressed in the heart，liver，kidney and skeletal muscle in G0，G1 mice，and the expression in G0 was higher than that in G1 mice.This study suggests that bovine FABP3 gene has been successfully integrated and expressed in the mouse DNA，and expressed in G1 mice.The result would help us guide beef cattle molecular genetic breeding in the future.

中图分类号:

S823
S813.3

李娟，杜新华，陈燕，高雪，李俊雅，许尚忠. 牛FABP3转基因小鼠的遗传及表达特性研究[J]. 畜牧兽医学报, 2015, 46(10): 1750-1758.

LI Juan，DU Xin-hua，CHEN Yan，GAO Xue，LI Jun-ya，XU Shang-zhong. Study on Genetic Characteristic and Expression of Bovine FABP3 Gene in Transgenic Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(10): 1750-1758.

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https://www.xmsyxb.com/CN/Y2015/V46/I10/1750

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